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. 2017 Sep 25;45(19):11327–11340. doi: 10.1093/nar/gkx813

Figure 1.

Figure 1.

Genetic and structural context of RNA:DNA hybrid-interacting region of RNAP β subunit. (A) Sequence alignment of 510–574 region (Escherichia coli numbering) of RNAP β subunit from various organisms. Amino acid residues whose mutations confer specific phenotypes are indicated. Dots represent identical residues; hyphens mark deletions. S531 or equivalent residues in various sequences are boxed and indicated. (B) The scheme for relative arrangement of nucleic acid scaffold, active center, HBS and rifampicin-binding site in TEC. (C) Stereo view for the surrounding of Rif compound bound to RNAP (PDB code: 1I6V). Bound drug (green) and neighboring amino acid chains are shown in a spacefil rendition. Side chains forming a cavity in the rifampicin binding pocket (element colored) and S531 residue (pink) are indicated. (D) the structure of RNAP TEC. (E) Magnified view of the RNAP region that interacts with RNA transcript in RNA:DNA hybrid (HBS) of the TEC. β subunit residues of HBS contacting RNA are shown in spacefil mode. RNA (green) is in stick representation. Structures are from (42) for (D) and 2O5J for (E).