Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 25;45(19):11327–11340. doi: 10.1093/nar/gkx813

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — Effect of S531 and R529 substitutions of abortive dinucleotide synthesis by RNAP. (A) Top, initial transcribed sequence of T7A2 promoter. Bottom, phosphoimage of the reaction products (for multi-round pppGpC synthesis) separated by ion-exchange TLC. (B) Quantitation of the results of (A). (C) Performance of the 531-D and -E in multiple- and single-round reactions of dinucleotide synthesis. (D) Effect of 529 substitutions on RNAP abortive dinucleotide synthesis. (E) Proposed mechanism for the influence of 531-D and -E substitutions on dinucleotide synthesis. The structure used for modeling is2O5J. The error bars represent SD from three independent experiments.