Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 25;45(19):11327–11340. doi: 10.1093/nar/gkx813

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 3. — Catalytic properties of 531 and 529 RNAP variants in in vitro transcription assays. (A) The image of radioactive RNA products of initiation after electrophoretic separation. (B) Quantitation of the results of (A). (C) Performance of 529 substitutions in the conditions of (A). (D) Quantitation of (C). (E) Elongation properties of 529 mutants. (F) The mechanism proposed to explain the positive effect of 531-R and -K substitutions on promoter clearance. Error bars represent SD from three independent experiments.