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. 2017 Sep 25;45(19):11281–11294. doi: 10.1093/nar/gkx839

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Protospacers are enriched around CRISPR arrays. (A) Cartoon model showing steps of CRISPR array expansion during acquisition of new spacers. The protospacer 3′-OH group carries out a nucleophilic attack on the leader repeat junction and the repeat-first spacer junction. The protospacer is integrated into the CRISPR array and the array is expanded by a unit of a new spacer and repeat. (B and C) Protospacers on the plus and minus strand are indicated in blue and pink respectively. Protospacers were deduced by aligning new spacers acquired into the CRISPR7 locus of WT and OE strains. New spacers acquired into other CRISPR arrays showed similar trends. Black bars show areas were protospacers are significantly enriched, as detected by peaking finding software in the HOMER package. (B) Protospacers are significantly enriched around the CRISPR6 array. (C) More subtle enrichment was observed around CRISPR2 and CRISPR4 arrays.