Skip to main content
. 2017 Aug 30;45(18):10751–10763. doi: 10.1093/nar/gkx744

Figure 5.

Figure 5.

AtPolIs bypass an apurinic/apyrimidinic (AP) site. (A) Time-course nucleotide incorporation reaction, from 15 s to 1 min, showing primer extension by AtPolIA, AtPolIB and AtPolIB exo−, in comparison to KF-DNAP I in canonical (lanes 1–9) or damaged templates (lanes 10–21) in the presence of 0.1 nM DNAP and 2 nM DNA. The template strand used for primer extension contained a non-damaged base or an AP site (tetrahydrofuran) 6 nts after the 3′-OH end of the primer. The migration of the 21-mer substrate and full-length extension 59-mer product are indicated in the gel. (B) Graphical representation of the lesion bypass efficiency by AtPolIs in comparison to KF-DNAP I. The values represent the relative amounts of diverse DNAs present in the reactions after 1 min. The products are subdivided into three classes: approach (before the AP site), insertion (incorporation opposite the AP site) and extension (nucleotide incorporation pass the AP site). In this representation the total amount of substrate and products is equal to 100%. Data represent the mean of three independent experiments.