Table 1. Kinetic parameters for nucleotide insertion and extension by KF-AtPolIB exo⁻ opposite canonical and AP sites.

Template	Incoming nucleotide	K M (μM)	k cat (min−1)	k cat/KM	f cat relative thymine-dATP
Insertion opposite T
	dATP	0.7605 ± 0.15	1.9722 ± 0.04	2.59 ± 0.26	1
	dTTP	261.6 ± 59	0.3019 ± 0.02	1.154 × 10−3 ± 0.003	4.45 × 10−4 (↓ 2247 x)
	dGTP	24.8 ± 5.1	0.37 ± 0.01	1.48 × 10−2 ± 0.003	5.74 × 10−3 (↓ 174 x)
	dCTP	185.3 ± 38.8	0.11545 ± 0.01	6.23 × 10−4 ± 0.0004	2.40 × 10−4 (↓ 4166 x)
Insertion opposite AP site
	dATP	7.2 ± 0.57	1.464 ± 0.04	0.202 ± 0.0633	0.08
	dTTP	40.8 ± 2.99	0.8248 ± 0.015	2.02 × 10−2 ± 0.004	7.7 × 10−3 (↓ 10 x)
	dGTP	51.95 ± 3.52	0.9973 ± 0.02	1.91x 10−2 ± 0.004	7.3 × 10−3 (↓ 11 x)
	dCTP	186.9 ± 30.9	0.2071 ± 0.025	1.11 × 10−3 ± 0.0004	4.2 × 10−4 (↓ 190 x)
Extension with dGTP (X)
	A-AP site (X)-C	18.41 ± 2.32	0.184 ± 0.006	1.0 × 10−2 ± 0.0024	3.9 × 10−3