Table 1.

Alignment of transmembrane P2X4R, P2X2R, and P2X7R rat sequences.

	Receptor	Sequence
TM1		42 46    50
	ratP2X4R	28VGLMNRAVQLLILA$\text{Y}$VIG$\text{W}$VFV$\text{W}$EKGY54
	ratP2X2R	28LGFVHRMVQLLILL$\text{Y}$FVW$\text{Y}$VFIVQKSY54
	ratP2X7R	28YGTIK$\text{W}$ILHMT$\text{V}$FS$\text{Y}$VS-$\text{F}$ALMSDKLY53
TM2		336     341       349
	ratP2X4R	331$\text{D}$IIPT$\text{M}$I$\text{N}$VG$\text{S}$$\text{G}$LAL$\text{L}$$\text{G}$V$\text{A}$TVLCDV$\text{I}$VL358
	ratP2X2R	331$\text{S}$LIPT$\text{I}$I$\text{N}$LA$\text{TA}$LTS$\text{IG}$VGS$\text{F}$LCD$\text{W}$$\text{I}$LL358
	ratP2X7R	331$\text{D}$IIQL$\text{V}$V$\text{Y}$IG$\text{ST}$LS$\text{Y}$$\text{FG}$L$\text{A}$TVCIDL$\text{I}$IN358

Key amino acids previously reported to participate in P2X4R IVM modulation are written in red, showing the coincidence with the present residues obtained by molecular docking (gray boxes). The corresponding residues in P2X2R and P2X7R, enumerated from the alignment with the P2XR sequence, are shown in orange.