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. 2017 Oct 26;4(12):1700401. doi: 10.1002/advs.201700401

Figure 4.

Figure 4

BMSCs cultured in TSSP, 1CSP, 2CSP, 3CSP, and 4CSP‐AKT bioceramic scaffolds for different time periods. a,b) SEM images of BMSCs attached in the channels of biomimetic scaffolds after culturing for 3 d. b) BMSCs adhered on the scaffolds via numerous filopodia as shown by the yellow arrows. c–e) The CLSM images for the morphology and cytoskeleton of BMSCs on the surface of struts and channels in TSSP, 1CSP, 2CSP, 3CSP, and 4CSP scaffolds after culturing for 3 d. d) Surface magnified image and e) 3D image shows that BMSCs penetrated into channels and attached on the inner walls of channels. f) The amount of adhered BMSCs after 4, 8, 16, and 24 h culturing and g) the proliferation activity of BMSCs in different scaffolds after 1, 3, and 7 d of incubation respectively, detected by the CCK‐8 assay. The initial adhered cells and their proliferation activity enhanced with the increase of the channel numbers in the biomimetic scaffolds. (n = 6, **P < 0.01, ***P < 0.001.)