Figure 2.

The occurrence of Pfam domain families in splicing factors. The known RNA splicing factors are annotated in UniProt according to the Pfam domain families of the protein domains found in these factors. A splicing factor may have multiple domains that belong to multiple Pfam families, and a Pfam domain family may contain domains in multiple splicing factors. The Pfam annotations were retrieved for each of 353 splicing factors, and the number of splicing factors was calculated for each of the Pfam families. For the 56 splicing factors that have curated datasets in SFMetaDB, the number of splicing factors was also calculated for the associated Pfam families. In the dodged barplots, the Pfam domain families are ranked by the number of the splicing factors which contain domains in the given families. Of the total 217 Pfam domain families annotated in UniProt, 26 Pfam domain families have ≥3 splicing factors annotated. The Pfam domain family with the most number of splicing factors is Pfam RRM_1 (PF00076). It contains 87 splicing factors, and 25 of these splicing factors have been studied according to our curation results. However, the splicing factors in the rest of the Pfam domain families have brought relatively less attention in RNA-Seq analysis, and they may be promising candidates for future studies.