Figure 2.

The 5′ end of the ∼380-nt species that accumulates in a Δrny strain is distinct from that of the mature species (M). Primer extension of total RNA isolated from wt, Δrny, ΔrnjA strains and the ΔrnjA Pxyl-rny depletion strain grown in the presence (+) and absence (–) of inducer (ind). C2 cleavage sites are shown as red arrows, the mature species (M) as a black arrow and the Δrny specific 5′ end (M+4) as green arrow (n = 2). The sequence is labeled as its complement to facilitate direct readout.