Fig. 2.
Fractionation of Bacillus amyloliquefaciens UCMB5113 LP extract and antifungal tests. (A) RP-HPLC profile of LP extract obtained after acid precipitation of B. amyloliquefaciens UCMB5113 growth medium was obtained using a C18 reversed phase column using a water/acetonitrile/TFA gradient and the eluent monitored at 215 nm. (B) Growth inhibitory activity of the LP extract fractions isolated by RP-HPLC was tested on the phytopathogens (a) A. brassicicola, (b) A. brassicae, (c) B. cinerea, (d) S. sclerotiorum and (e) V. longisporum. HPLC fractions 1–14 or methanol as control (C) were added to different parts of the Petri dish and growth was studied 3–14 d later.