Fig. 5.

qPCR analysis of A. brassicicola levels in B. amyloliquefaciens UCMB5113 primed Arabidopsis plants. One-week-old seedlings of Arabidopsis wildtype or the mutants coi1-16, sid2, jar1, myc2, myb72 and npr1 were tested. Roots of one-week-old seedlings grown on MS media were treated with water, 5 % methanol or an LP extract. Two weeks after treatment, four leaves of each plant were inoculated with A. brassicicola. Five days after fungal inoculation, the level of Alternaria DNA was quantified by qPCR. ANOVA was run to compare DNA levels among the treatments for each plant line where different letters indicate significant differences. Plants were untreated (white bar), treated with methanol as solvent control (grey bar) or treated with LP extract (black bar).