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. 2017 Jun 29;45(14):8411–8422. doi: 10.1093/nar/gkx572

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Published by Oxford University Press on behalf of Nucleic Acids Research 2017.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 2. — ZFP-PB mediated gene targeting of HPRT. (A) four different ZFPs were engineered as described in the Materials and Methods to target HPRT. The approximate binding locations are depicted by the black triangles. (B) ChIP of the differing ZFP-PB chimeras at their cognate target sites in the human genome. HA-tagged PB, HA-PB, is depicted as a negative control. None represents the absence of transfected transposase. (C) G418 resistant colonies produced after co-transfection of the PB-SB-SA-βGeo plasmid with the varying ZFP-PB chimeras. ANOVA followed by Bonferroni post-test analysis revealed no PBase (no transposase) to be the only condition significantly different from HA-PB (the positive control) (N = 4, ±SEM). (D) Colony number after replating G418 resistant cells into and selecting with 6-TG. ANOVA with Bonferroni post-test revealed Z3-PB to be significantly different from HA-PB (N = 4, ±SEM).