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. 2017 Jun 29;45(14):8411–8422. doi: 10.1093/nar/gkx572

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Published by Oxford University Press on behalf of Nucleic Acids Research 2017.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 3. — TAL-PB mediated gene targeting of HPRT. (A) four different TALs were engineered with the approximate binding locations are depicted by the black triangles. (B) ChIP of the differing TAL-PB chimeras at their cognate target sites in the human genome. HA-PB, is depicted as a negative control. None represents the absence of transfected transposase. (C) G418 resistant colonies produced after co-transfection of the PB-SB-SA-βGeo plasmid with the varying TAL-PB chimeras. ANOVA followed by Bonferroni post-test analysis revealed no PBase (no transposase) to be the only condition significantly different from HA-PB (the positive control) (N = 4, ±SEM). (D) Colony number after replating G418 resistant cells into and selecting with 6-TG. ANOVA with Bonferroni post-test revealed TAL2-PB to be significantly different from HA-PB (N = 4, ±SEM).