Figure 2.

Binding of MobM to supercoiled pMV158 DNA. Plasmid pMV158 was incubated with MobM at 30°C for 30 min. MobM–pMV158 complexes were fixed with glutaraldehyde and then pMV158 was linearized with StuI. (A) Electron micrographs of linear plasmid molecules with MobM bound to site I or site II (black arrows). (B) Distribution of MobM positions on the pMV158 DNA when the binding reactions were performed in the presence of MgCl₂ or in its absence. The coordinates of the two main MobM-binding sites are indicated.