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. 2017 May 19;45(13):7774–7785. doi: 10.1093/nar/gkx450

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Fucose-mediated induction of mobM expression in pneumococcal cells. (A) Relevant features of plasmids pMV158, pMVT and pMVT-PfcsK: ssoU, lagging strand replication origin; oriT, origin of transfer; T1T2, tandem transcriptional terminators T1 and T2 of the Escherichia coli rrnB rRNA operon; Pmob, promoters of the mobM gene; PfcsK, promoter induced by fucose. (B) Western blot analysis using antibodies against MobM. Pneumococcal cells carrying plasmid were grown in media containing 0.3% sucrose and the indicated concentration of fucose. Total proteins were separated by sodium dodecyl sulphate (SDS)-polyacrylamide (12%) gel electrophoresis. Pre-stained proteins (Invitrogen) were run in the same gel as molecular weight markers (not shown). Purified MobM (400 ng) was run in the same gel.