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. 2017 May 19;45(13):7774–7785. doi: 10.1093/nar/gkx450

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Effect of MobM on the plasmid copy number. Total DNA was isolated from pneumococcal cells harbouring either pMVT (promoter-less mobM) or pMVT-PfcsK (mobM expression is induced by fucose; see Figure 5). Cells were grown in media containing (+) or not containing (−) fucose. Total DNA extracts (3, 6 and 12 μl) were analysed by agarose (0.8%) gel electrophoresis in the presence of ethidium bromide (1 μg/ml). Chromosomal DNA (chr), open circular plasmid DNA (FII) and covalently closed circular plasmid DNA (FI) are indicated. The average copy number of the plasmids (N_av) is indicated.