Fig. 1.

Flow-cytometric (FCM) estimates of relative genome size and confirmatory karyological analyses of Tripleurospermum inodorum. (A) Simultaneous FCM analysis of five individuals representing the five euploid cytotypes (2x–6x) distinguished during ploidy screening, with Bellis perennis as the internal standard. (B–D) Photomicrographs of metaphase chromosomes of individuals belonging to the three most common ploidy levels: diploid (2n = 18; B), triploid (2n = 27; D) and tetraploid (2n = 36; C). (E,F) Metaphase chromosomes of two individuals identified as putative aneuploids in FCM analyses, and later confirmed as being hypertriploid (2n = 29; E) and hypertetraploid (2n = 40; F), respectively.