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. 2017 Sep 5;45(19):11315–11326. doi: 10.1093/nar/gkx794

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Pol IV and Pol V have altered fidelities relative to Pol II. (A) Design of the assay. (B) Pol II, IV, and V primer elongation products visualized by phosphorimaging following electrophoresis on a 15% PAGE gel. (C) Effect of NTP: DNA template combination on Pol II, IV, or V misincorporation. (D) Primer elongation fidelity assay comparing Pol IV immunoprecipitated from a FLAG-tagged NRPD1 transgenic line in which RDR2 co-immunoprecipitates with Pol IV (Pol IV-FLAG), Pol IV immunoprecipitated from an rdr2 null mutant line (Pol IV-FLAG, rdr2–1), or Pol IV immunoprecipitated from a transgenic line expressing an active site mutant form of the NRPD1 subunit (ASM-FLAG). (E) Effect of manganese in the transcription assay, in place of magnesium.