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Effect of DNA template cytosine methylation on Pol II, IV or V transcriptional fidelity. (A) Overview of primer elongation assay. (B) Tests for effects of cytosine methylation in the CHH context (5′-CTT-3′ or 5′-CAA-3′) on ATP or UTP misincorporation. (C) Tests of cytosine methylation in the CG context (5′ to 3′) on CTP misincorporation.
