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. 2017 Sep 5;45(19):11315–11326. doi: 10.1093/nar/gkx794

Figure 5.

Figure 5.

Relative nucleotide incorporation rates of Pols II, IV and V. (A) Assay design. (B) Representative gels showing the incorporation of UTP by Pols II, IV and V versus time (UTP concentration = 500 nM). (C) Percent incorporation (intensity of elongation products divided by total products) versus time, with linear regression used to fit the curves to the equation c(t) = A × (1 – exp[–k × t]). (D) Elongation rate constants for Pols II, IV and V. Data is representative of two replicate experiments (see Supplementary Figure S4).