Figure 1.

Immunoblot of BER proteins captured by iPOND. HEK293 cells were pulsed with EdU for the indicated lengths and the negative control was mock-treated with DMSO. One sample was followed by a thymidine chase for additional 30 min after the EdU-pulse. The cells were crosslinked in formaldehyde (FA) (1%) before harvesting. The input samples contain protein of the cell lysate (100 μg, 0.13%). The capture samples contain protein from nascent DNA. All data are derived from the same biological replicate and from one gel.