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. 2017 Aug 21;45(19):11121–11130. doi: 10.1093/nar/gkx728

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Design of TALE-TF constructs. (A) Potential regulatory effect of transcription factor (TF) DNA residence time on gene transcription. Long DNA binding of a transcription repressor might lead to enhanced repression efficiency compared to short DNA binding due to more efficient competitive inhibition of an activator or recruitment of co-factors. (B) Domain structure of the TALE-based TFs. After a N-terminal HaloTag varying numbers of TALE DBD repeat domains recognizing the GR response element of SGK1 are inserted, followed by a C-terminal domain (AF-2 of GR or VP64) or no domain (⊘). (C) U2-OS cells expressing GRc16R labeled with TMR in the absence (left panel) and presence (right panel) of 1 μM Dex.