Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 6;45(14):e130. doi: 10.1093/nar/gkx504

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — OFF- and ON-states of FASTmiR122 and FASTmiR171. (A) The predicted structure of the FASTmiR122 OFF-state and the ON-state with the miR122 target. The OFF-state contains a disrupted base triple and G-quadruplex that forms two small hairpin structures (arrowheads). Free DFHBI (gray) cannot bind and then fluoresce. The miR122 (green) binds and opens the structure, resulting in a re-formed modSpinach (right of black arrowheads) that can bind DFHBI and then fluorescence. The FASTmiR122 ON-state bound by miR122 is favored thermodynamically (MFE = −52.90 kcal/mol) compared to the unbound OFF-state (MFE = −31.10 kcal/mol. ΔG binding =−21.81 kcal/mol). (B) The predicted structure of the FASTmiR171 OFF-state and the ON-state with the miR171 target. The G-quadruplex of the OFF-state is disrupted by one hairpin structure and one paired region (arrowheads). The OFF sequence has two toe-hold-like regions where the miR171 (green) can enter, bind and open the OFF-state structure. The FASTmiR sensor then switches to the ON-state that has a reformed modSpinach (right of arrowhead) that can fluoresce. The FASTmiR171 ON-state bound by miR171 is favored thermodynamically (MFE = 57.10 kcal/mol) compared to the unbound OFF-state (MFE =−29.7 kcal/mol. ΔG binding= −25.14 kcal/mol). The OFF-states in A and B are drawn based on predicted structure from Vienna RNA secondary structure server. MFE and ΔG binding were calculated using Vienna RNAfold and RNAcofold (version 2.1.1). The ON-states in A and B are drawn according to Huang et al. (18).