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. 2017 Jun 16;45(14):8392–8402. doi: 10.1093/nar/gkx532

Figure 3.

Figure 3.

Both hypusinated and unhypusinated eIF5A substitute for polyamines to stimulate general translation. (A) Maximum fractions of MFFF peptide synthesis obtained in polyamine-deficient in vitro reconstituted translation assays supplemented with the indicated amounts of spermidine, spermine or putrescine (structures depicted above plots) were plotted and fit to the Michaelis–Menten equation. K1/2(endpoint) is the polyamine concentration at which 50% of the Ymax is obtained. (B, C) Maximum fractions of MFFF (B) and MPPPK (C) peptide synthesis obtained in polyamine-deficient in vitro reconstituted translation assays supplemented with no eIF5A (No), 5 μM wild type hypusinated eIF5A [WT(+Hyp)], or three versions of eIF5A (5 μM) lacking hypusine: wild type eIF5A (K51), eIF5A-K51A, or eIF5A-K51R, as indicated (see also Supplementary Figure S3). Error bars are SD from at least three independent experiments (B, C).