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. 2017 Aug 16;45(19):11056–11069. doi: 10.1093/nar/gkx721

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — p53 regulation of APOBEC3B expression is mediated by p21 acting through the E2F4 DREAM transcriptional complex. (A) Shown is a model depicting the mechanism by which the DREAM complex regulates gene expression following p53 activation. (B) RT-qPCR for p21-null HCT116 cells treated with Nutlin for 24 h. (C) RT-qPCR of HCT116 cells transfected with p21 siRNA. Nutlin was added for 24 h. Significant (P < 0.05; n = 3) differences between vehicle and Nutlin-treated cells are highlighted by asterisks. (D) Immunoblotting of HCT116 cell lysates following p21 knockdown. (E and F) RT-qPCR and immunoblotting of HCT116 cells transfected with siRNA for E2F4.