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. 2017 Aug 25;45(18):10824–10836. doi: 10.1093/nar/gkx734

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Negative staining electron microscopy images of Nob1p-containing particles obtained by tandem affinity purification from (A) TetO7-RIO1, NOB1-FPZ cells grown in YPD without doxycycline (Rio1p expressed), or (B) TetO7-RIO1, NOB1-FPZ cells grown in YPD plus doxycycline for 16 h (Rio1p depleted). Putative pre-40S particles (40S-like particles), 80S-like particles and polysomes are highlighted as indicated below the images. (C) Percentage of 40S-like, 80S-like particles and putative polysomes in Rio1p-expressing and Rio1p-depleted cells. (D) Examples of polysomes seen in images obtained from TetO7-RIO1, NOB1-FPZ cells grown in YPD plus doxycycline.