Table 1. Oligonucleotides used.
2XFlag FW | 5′CATGGACTACAAGGACGACGATGACAAAGGTACCGATTACAAAGATGATGACGACAAAG3′ |
2XFlag Rev | 5′CTAGCTTTGTCGTCATCATCTTTGTAATCGGTACCTTTGTCATCGTCGTCCTTGTAGTC3′ |
PPX_FW | 5′CTAGCCTGGAAGTTCTGTTCCAGGGGCCCGAG3′ |
PPX_REV | 5′CTCGGGCCCCTGGAACAGAACTTCCAGG3′ |
Nob1 MFM Tag-Nat-Fd | 5′ACGTCCGCATTGGTAAGGGAAGGTACGTCAACAGTTCCAAAAGGAGAAGTTCCATGGACTACAAGGACGAC3′ |
Nob1 MFM Tag-Nat-Rev | 5′TCTTCAAAGTGCTGTAACACCATTCATCTCATAAGGGGAGGGCATACTGAGCAATTACGACTCACTATAGG3′ |
Nob1–1081 FD | 5′AACAGATATTCTGTAGCCAG3′ |
tRNAiMet probe | 5′TCGGTTTCGATCCGAGGACATCAGGGTTATGA3′ |