Figure 1.

Motif 1 is required for RP gene transcription in cells. (A) Schematic of the RP gene luciferase reporter assay. Mutant RpLP1 and RpL30 have three highly conserved nucleotides in Motif 1 mutated. The wt and mutant Motif 1 sequences for both promoters are shown below the illustration. The RpIII128 promoter lacks Motif 1 and serves an internal control. (B) Firefly/Renilla luciferase ratio of relative light unit measurements. Ratios are normalized to the wt Motif 1 sample for each promoter. Error bars represent standard deviation (n = 3).