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. 2017 Sep 7;45(19):11425–11436. doi: 10.1093/nar/gkx795

Figure 4.

Figure 4.

Redox regulation of FosB/JunD in vitro. (A) Comparison of the FosB and JunD monomers in the FJoxi and FJ-DNA crystal structures. (B) Redox titration of the FosB/JunD bZIP. Error bars represent standard deviations for three replicates. Inset shows the abundance of Arg/Lys residues within 10 Å of the redox-sensitive C172 (FosB) and C285 (JunD) in FJoxi. Color scheme is as indicated in Figure 3. (C) Circular dichroism spectra of 0.1 mg/ml (6 μM) FJoxi in the absence and presence of 1 mM TCEP and/or AP-1 DNA at equal molar. Dotted lines indicate 0% and 100% helicity, respectively. (D) Titration of FJoxi heterodimer into 10 nM AP-1 DNA labeled with TAMRA at both 5′-ends in the absence and presence of 1 mM DTT monitored by fluorescence polarization. Error bars indicate the standard deviation for two replicates.