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. 2017 Jun 28;45(14):8635–8645. doi: 10.1093/nar/gkx567

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Stimulation of integrase activity by RDF is intramolecular. (A) Hypothetical intra-subunit or inter-subunit activating interactions of the RDF and integrase moieties of an integrase–RDF fusion protein. The diagrams represent the intermediate synaptic complex formed when two integrase-bound att sites interact. Only one RDF moiety is shown in each diagram, for clarity. The stimulated integrase subunit is in blue, and the subunit donating the RDF moiety (where different) is in brown. (B) ϕC31 integrase (400 nM) was added to pϕC31LRX substrate and the mixture was incubated at room temperature for 5 min to allow binding and full occupation of the sites; then gp3 (lane 3), ϕC31.Int(S12A)-gp3 (lane 4), or Bxb1.Int-gp3 (lane 5) was added. The mixture was then incubated for 1 h at 30°C, and the products were detected as in Figure 3. Mean extent of recombination and standard deviation (%) from quantitation of triplicate experiments are given below relevant lanes.