Table 1. Primers for generating nucleosomal DNA-templates.
Forward primer (5′-3′): | Reverse Primer (5′-3′): | Symmetrically positioned mononucleosomes |
atc ttt tga ggt ccg gtt ctt t | gta cag aga ggg aga gtc aca aaa c | 77-NPS-77 |
atg ttt ggg cc acct ccc c | taa cgg cct taa gag aaa ttt ct | 40-NPS-40 |
gat cca gaa tcc tgg tgc tga g | tag ctg tat ata tct gac aca tg | 0-NPS-0 |
Forward primer (5′-3′): | Reverse Primer (5′-3′): | Asymmetrically positioned mononucleosomes |
gat cca gaa tcc tgg tgc tga g | gta cag aga ggg aga gtc aca aaa c | 0-NPS-77 |
ggccgccctggagaatc | Cy3-gcgtatagggtccatcacataacc | 6-NPS-47 (pUC18 12x-601) assembly with human octamers |
Forward primer (5′-3′): | Reverse Primer (5′-3′): | Dinucleosome |
gcgaattggagctccaccgc | ggaattcctgcagaccgggg | 601-10N-601 |