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. 2017 Aug 28;45(18):10534–10554. doi: 10.1093/nar/gkx711

Table 1. Primers for generating nucleosomal DNA-templates.

Forward primer (5′-3′): Reverse Primer (5′-3′): Symmetrically positioned mononucleosomes
atc ttt tga ggt ccg gtt ctt t gta cag aga ggg aga gtc aca aaa c 77-NPS-77
atg ttt ggg cc acct ccc c taa cgg cct taa gag aaa ttt ct 40-NPS-40
gat cca gaa tcc tgg tgc tga g tag ctg tat ata tct gac aca tg 0-NPS-0
Forward primer (5′-3′): Reverse Primer (5′-3′): Asymmetrically positioned mononucleosomes
gat cca gaa tcc tgg tgc tga g gta cag aga ggg aga gtc aca aaa c 0-NPS-77
ggccgccctggagaatc Cy3-gcgtatagggtccatcacataacc 6-NPS-47 (pUC18 12x-601) assembly with human octamers
Forward primer (5′-3′): Reverse Primer (5′-3′): Dinucleosome
gcgaattggagctccaccgc ggaattcctgcagaccgggg 601-10N-601