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. 2017 Sep 13;45(18):10380–10392. doi: 10.1093/nar/gkx803

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — (A) Absorption and (B) emission spectra of DFHBFSI (10 and 3 μM) upon stepwise addition of NG16. Inset of B is plot of fluorescence enhancement ratio (F/F₀) versus the concentration of NG16. (C) Changes in CD spectra of NG16 (10 μM) upon the addition of DFHBFSI (50 μM) in different buffer conditions. K⁺, 100 mM; Mg²⁺, 50 mM. (D) CD melting curves of NG16 (5 μM) at 265 nm under different conditions. NG16 (curve 1) and NG16 with DFHBFSI (25 μM, curve 2) in buffer without metal ions; NG16 (curve 3) and NG16 with DFHBFSI (25 μM, curve 4) in buffer containing K⁺ (100 mM) and Mg²⁺ (50 mM).