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. 2017 Jun 6;24(5):537–548. doi: 10.1093/dnares/dsx023

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© The Author 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Extraction of genomic regions interacting with the Pax5 promoter region in DT40. (Step 1) Removal of off-target binding sites. iChIP-Seq data were compared between KI(B) and Non-KI(B) (negative control) using Model-based Analysis of ChIP-Seq (MACS) to eliminate off-target binding sites. (Step 2) Analysis of another biological replicate of Step 1. (Step 3) Identification of genomic regions that interact with the Pax5 promoter region. The genomic regions commonly detected in Steps 1 and 2 represent candidate genomic regions that physically interact with the Pax5 promoter region.