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. 2017 Jun 6;24(5):537–548. doi: 10.1093/dnares/dsx023

Figure 6.

Figure 6.

A genomic region interacting with the Pax5 promoter is involved in transcriptional regulation of the Pax5 gene. (A) Schematic depiction of the loci in chromosome 11 that were identified as interacting with the Pax5 promoter region in a B cell-specific manner. One allele is shown. The interacting regions are shown as gray triangles. (B) CRISPR-mediated knock-out of the interacting regions in DT40. (C) Primer sets for evaluation of the amounts of Pax5 mRNA. (D) Expression levels of the Pax5 gene in the knock-out cells. Expression levels of Pax5 were normalized to those of GAPDH, and the mRNA levels in the control cells were defined as 1 [mean ± s.e.m., n = 3 (two upper graphs), n = 6 (others)]. WT: DT40. *: t-test P value < 0.05. all: mRNA transcribed from both the Pax5 exons 1A and 1B, 1A: mRNA transcribed from the Pax5 exon 1A, 1B: mRNA transcribed from the Pax5 exon 1B.