Table 3. The effects of transcriptional inhibition on the accumulation of Cp small RNA and RNA.
| Cp region | WTSS (RPM × 103) | sRNA-Seq (RPM × 103) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mixotrophic | Phototrophic | Mixotrophic | Phototrophic | ||||||||||
| Length (∼kb) | Type | T0 | Rif 6 h | log2FC | T0 | Rif 6 h | log2FC | T0 | Rif 6 h | log2FC | T0 | Rif 6 h | log2FC |
| 21 | 5′UTRs | 43±8 | 17±5 | -1.2 | 46±9 | 7.7±0.8 | -2.6 | 1.9±0.1 | 0.5±0 | -1.8 | 0.9±0 | 0.4±0 | -1.3 |
| 88 | CDS | 542±67 | 226±48 | -1.2 | 573±61 | 169±16.5 | -1.8 | 2±0 | 0.4±0 | -2.3 | 1.4±0.1 | 1.0±0.1 | -0.5 |
| 13 | 3′UTRs | 32±8 | 13±4 | -1.2 | 34±6 | 8.0±0.2 | -2.1 | 0.8±0 | 0.1±0 | -2.3 | 0.5±0 | 0.1±0 | -1.9 |
| 0.1 | Introns | 0.2±0 | 0.05±0 | -2.2 | 0.27±0 | 0.03±0 | -3.0 | 0.0±0 | 0.0±0 | -2.9 | 0.0±0 | 0.0±0 | -1.9 |
| 40 | Intercistronic | 42±6 | 12±3 | -1.7 | 47±8 | 7.2±0.5 | -2.7 | 5.2±1 | 1.8±0 | -1.4 | 4±1 | 2.3±0.3 | -1.0 |
| 2 | mature tRNA | 28±6 | 11±3 | -1.4 | 28±5 | 7.0±0.1 | -2.0 | 49±13 | 31±0.5 | -0.6 | 31±3 | 38±4 | 0.3 |
| 5 | mature rRNA | 7±0.7 | 1.5±0.3 | -2.2 | 6±0.7 | 1.5±0.6 | -2.2 | 28±4 | 1.9±0.2 | -1.1 | 28±0.5 | 18±1 | -0.6 |
sRNA-Seq and WTSS coverage, reported as the sum of the reads normalized to the total mapped to the nuclear and mitochondrial genomes for each Cp region and averaged between two biological replicates (RPM±SD). Introns are for psaA. The inverted repeat A, psbA and intercistronic regions between convergent gene units are excluded. The log2 Fold-Change (FC) between Rif- and control is indicated.