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. 2017 Jul 29;45(18):10436–10451. doi: 10.1093/nar/gkx667

Figure 3.

Figure 3.

RNA editing/dsRNA binding-independent suppression by ADARs on METTL7A expression in HCC cells and primary tumors. (A) Western blot analyses of the indicated proteins in Huh-7 cells 72 h after transfection with the wild-type, DeAD or EAA mutants of ADARs (left panel). The bar chart represents the normalized densitometry unit of METTL7A protein on Western blot (right panel). (B) qRT-PCR analysis of METTL7A mRNA level measured in the same samples as described in (A). (C) Western blot analyses of the indicated proteins SMMC7721 cells 72 h post-transfection with shRNAs against either ADAR1 or ADAR2. Value indicates the normalized densitometry unit of METTL7A protein on western blot. (D and E) Western blot analyses of METTL7A, ADAR1 and ADAR2 proteins in two groups of HCC cases including ADAR1/2 high (D) and ADAR1/2low or normal (E). Tubulin is the loading control. T, primary HCC tumor; NT, matched non-tumor liver. Data are presented as the mean ± S.E.M. of six replicates from a single experiment and representative of three independent experiments (A, B). Statistical significance is determined by unpaired, two-tailed Student's t test (A, B). (**P < 0.01; ***P < 0.001).