Figure 4.

Silencing activity of amide-modified siRNAs. Duplexed siRNAs, formed from the respective guide strands 5′-UCAGUUUGAAGUUCUCAUCUU-3′ (brown sequences in Figure 1) and the unmodified passenger strand 5′-GAUGAGAACUUCAAACUGAUU-3′, at 1 nM final concentration were transfected in HeLa cells using DharmaFECT 1 transfection reagent for 48 h. The level of knockdown was assessed using a branched DNA (bDNA) assay (QuantiGene branched DNA signal amplification kit; Panomics). UnMod = unmodified, matched sequence siRNA, PPIB = transfection control siRNA targeting PPIB (different target sequence), NTC = non-targeting control siRNA.