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. 2017 Aug 24;45(18):10837–10844. doi: 10.1093/nar/gkx748

Figure 1.

Figure 1.

CrPV-1A inhibits the target cleavage reaction by Ago2-RISC. (A) A scheme for the mode of action of representative VSSs during the multi-step pathway of RNA silencing. (B) The genome structure of cricket paralysis virus. The silencing suppressor protein 1A is encoded at the N-terminal of ORF-1 (red rectangle). Hel: helicase, pro: protease, RdRP: RNA-dependent RNA polymerase, VP: viral structural ptotein. (C) Target cleavage assay in crude lysate from S2 cells. As previously reported, CrPV-1A, but not DCV-1A or FHV-B2, inhibited the cleavage reaction. The recombinant proteins were added at the concentration of 0.35 μM. (D) The experimental procedure of the target cleavage assay by immunopurified Ago2-RISC in E. (E) Target cleavage assay by immunopurified Ago2-RISC. The recombinant proteins were added at the concentrations of 0.05–1.0 μM. (F) Quantification of the cleavage assay in E. Error bars indicate the SD (standard deviation) from three independent experiments.