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. 2017 Aug 24;45(18):10837–10844. doi: 10.1093/nar/gkx748

Figure 2.

Figure 2.

CrPV-1A inhibits target binding by Ago2-RISC. (A) The experimental procedure of the target binding assay by immunopurified Ago2-RISC in C and F. (B) The guide-target configuration used in C. The base pairing was perfectly complementary between the guide strand and the target RNA. (C) CrPV-1A was added at the concentrations of 0.01–1.0 μM in the target binding assay. Mock indicates mock immunopurification from naive S2 lysate. (D) Quantification of the binding assay in C. Error bars indicate the SD from three independent experiments. (E) The guide-target configuration used in F. The base pairing was complementary only in the seed region (guide positions 2–8) between the guide strand and the target RNA. (F) CrPV-1A was added at the concentrations of 0.01–1.0 μM in the target binding assay. Mock indicates mock immunopurification from naive S2 lysate. (G) Quantification of the binding assay in E. Error bars indicate the SD from three independent experiments.