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. 2017 Sep 13;45(19):11268–11280. doi: 10.1093/nar/gkx823

Figure 4.

Figure 4.

(A) Bar graphs indicate increased promoter activity of the genotype B wild-type preS2/S promoter (GTB-luc-WT) as compared to the mutated genotype B preS2/S promoter (GTB-luc-MUT) as measured using luciferase reporter constructs. The luminescence from pRL-TK, which has a thymidine kinase promoter upstream of the Renilla luciferase CDS served as an internal control for normalization. CD melting showing stabilization of the wild-type PQS (GTB-WT-PQS) oligonucleotide in the presence of (B) BRACO19 (10 μM) and (C) pyridostatin (PDS) (10 μM). ΔTm = Tm (with ligand) – Tm (without ligand). Melting was monitored at a wavelength of 292 nm. (D) Bar graph showing enhanced promoter activity of the genotype B wild-type preS2/S promoter (GTB-luc-WT) in the presence of BRACO19 as measured using luciferase reporter constructs (E) Bar graph showing enhanced promoter activity of the genotype B wild-type preS2/S promoter (GTB-luc-WT) in the presence of 10 μM pyridostatin (PDS) as measured using luciferase reporter constructs. Data are presented as mean ± SD (n = 4 replicates).