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. 2017 Sep 13;45(19):11268–11280. doi: 10.1093/nar/gkx823

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — (A) The HBV covalently closed circular (cccDNA) levels are comparable in Huh7 cells transfected with the full-length genotype B wild-type construct (GTB-full-WT; the preS2/S promoter of this construct has an intact G-quadruplex motif) and the full-length genotype B mutant construct (GTB-full-MUT; the preS2/S promoter is mutated to disrupt the G-quadruplex motif). The HBV cccDNA levels were measured using real-time PCR. Data are presented as mean ± SD (n = 4 replicates). (B) Northern blot analysis of HBV RNA from Huh7 cells transfected with GTB-full-WT and GTB-full-MUT constructs. The 3.5 kb HBV transcripts and the 2.4 kb HBV transcripts are comparable between GTB-full-WT and GTB-full-MUT. The GTB-full-MUT is associated with lower levels of the preS2/S transcript (2.1 kb) as compared to the GTB-full-WT. Actin mRNA is used as an internal control for northern blot analysis. Bar graphs showing densitometry analysis of (C) the 3.5 kb HBV transcripts (HBV pg RNA and pc RNA) and (D) the 2.1 kb transcript in the northern blot. Data are presented as mean ± SD (n = 3 replicates). Relative expression levels of (E) HBV pregenomic RNA and (F) HBV precore (pc RNA) using real-time quantitative PCR. Data are presented as mean ± SD (n = 4 replicates)