Figure 8.

Virion secretion from Huh7 cells transfected with full-length HBV constructs. The full-length genotype B wild-type construct (GTB-full-WT; the preS2/S promoter of this construct has an intact G-quadruplex motif) is associated with significantly higher levels of hepatitis B virion secretion as compared to full-length genotype B mutant construct (GTB-full-MUT; the preS2/S promoter is mutated to disrupt the G-quadruplex motif). Virions were estimated from the supernatants using an immunocapture-based method described in the methods section. Data are presented as mean ± SD (n = 3 replicates)