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. 2017 Aug 31;45(19):11341–11355. doi: 10.1093/nar/gkx764

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Association of Sla1p and hLa with modified or unmodified pre-tRNAs in vitro. (A): EMSA of Sla1p with T7 transcripts corresponding to candidate pre-tRNAs identified as having impaired charging in vitro (tRNA-Ser^UGA) or impaired accumulation (tRNA-Leu^UAG & tRNA-Leu^CAG) in the absence (unmodified) or presence (Trm1p modified) of G26 modification. (B) EMSA of hLa with a T7 transcript corresponding to mature Saccharomyces cerevisiae tRNA-Phe^GAA or its fully modified counterpart purified from S. cerevisiae cells.