Figure 4.

Arb1 depletion induces transcriptional elongation changes in the ribosome-related genes. (A) Comparison made between the standardised total RNA pol II occupancy (RPCC) and transcriptionally active RNA pol II (GRO) values (see the Materials and Methods) during Arb1 depletion. Arb1-depletion experiments were performed under the conditions defined in Supplementary Figure S6 using the arb1Δ TEToff::ARB1::MYC SPT6 strain. The samples taken before and after effective Arb1 depletion were compared. All the genes (blue), the RP regulon (red) and the RiBi regulon (orange) are indicated. (B) Heat map of the RPCC and GRO change during depletion for the RP genes and for the different subgroups present in the RiBi regulon. Fold-change of the z-scores (Arb1-depleted versus control) is indicated by the color bar as log₂ values. Data are from the experiments described in A (arb1Δ SPT6 TEToff::ARB1::MYC strain) and in Supplementary Figure S8C (arb1Δ TEToff::ARB1::MYC spt6–140 strain). Genes were ranked according to the RPCC/GRO ratio variation in SPT6 to visualize the impact of spt6–140.