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. 2017 Jun 15;45(16):9302–9318. doi: 10.1093/nar/gkx529

Figure 5.

Figure 5.

Spt6 recruitment upon Arb1 depletion prevents histone loss. (A) The arb1Δ TEToff:ARB1:MYC strain was transformed with an Spt6-HA-expressing centromeric plasmid to measure Spt6 occupancy during Arb1 depletion. The DNA regions located upstream of the transcription start sites and inside the ORFs of various RiBi genes were tested. (B and C) The occupancy of histone H3 (B) and RNA pol II-specific subunit Rpb3 (C) was measured by ChIP in the arb1Δ TEToff::ARB1::MYC strains (that contained either the SPT6 or the spt6–140 allele) at the indicated conditions. Both IPs came from the same extracts. ChIP values in relation to input. The spt6–140/SPT6 ratios are represented for Arb1-depleted and control conditions. The average and standard deviation of three biological replicates are shown. Two-tailed t-test; *P < 0.05, **P <0.005.