Figure 6.

Spt6 contributes to the synthesis of stable mRNA of ribosome biogenesis genes upon Arb1 depletion. (A) Fold-change of the mRNA levels of the five genes tested in Figure 5 upon Arb1 depletion, mRNA levels were measured by RT-PCR. (B) Fold-change of the mRNA half-lives of the same set of genes, upon Arb1 depletion. The mRNA half-lives were calculated after performing transcription shut-off experiments (see Materials and Methods). All the values represented as a fold over the SPT6 non-depleted control. The average and standard deviation of three biological replicates are shown. See Supplementary Figure S10 for the spt6–140/SPT6 ratios under the Arb1-depleted and control conditions.