Table 4. A summary of Stx1-containing Escherichia coli signatures generated by Neptune relative to a background of non-toxigenic Escherichia coli. The signatures were mapped to E. coli O157:H7 str. Sakai reference (NC_002695.1, NC_002127.1, NC_002128.1) to infer annotations.

Rank	Score	Length	Locus Information	E. coli O157:H7
		(bp)		Sakai coordinates
1	1.00	1375	Shiga toxin (A and B subunit)	2 924 383–2 925 757
2	0.99	5433	Urease gene cluster: ureA-G	1 390 114–1 395 545
3	0.98	3291	Bacteriophage related, integrase and other	2 593 022–2 596 313
4	0.98	438	perC, transcriptional activator of EaeA/BfpA, partial	1 183 201–1 183 639
5	0.98	1223	Phage tail length tape measure protein, partial	2 170 250–2 171 473
6	0.97	7697	Hemolysin gene cluster: hylC, hylA, hylB, hylD	15 716–23 412 (pO157)
7	0.96	1260	Colonization factor	1 767 898–1 769 157
8	0.96	962	Hypothetical	2 200 204–2 201 165
9	0.96	495	Hypothetical	2 186 120–2 186 614
10	0.96	796	Phage origin, serine/threonine protein phosphatase	3 488 405–3 489 201
11	0.96	1364	Hypothetical, colicin-like and small toxic polypeptide	1 397 029–1 398 393
12	0.96	987	Hypothetical, putative membrane protein	3 486 570–3 487 557
13	0.95	916	Putative serine acetlyltransferase of prophage	2 605 160–2 606 076
14	0.95	300	Hypothetical, potential T3SS effector	2 209 466–2 209 765
15	0.95	1136	T3SS effector protein NleH	1 804 974–1 806 122