Table 3.

Toxoplasma gondii Fatty Acid Beta Oxidation Enzymes, Catalase, Orthologs from Other Apicomplexans and Predicted Peroxisomal Targeting Signals

Enzyme	Tg	Nc	Et	Cc	Hh	Sn	Pf	Cp	Gn
FACS	+/PTS1 (AKL)	+/PTS1 (AKL)	+/PTS1 (CKL)	+/PTS1 (AKL)	+/PTS1 (AKL)	+/PTS1 (SKL)	+/no PTSa	+/SP, PTS1a	+/no PTSa
ACOX	+/PTS1 (SKL)	+/PTS1 (CKL)	+/PTS1 (PKL)	+/no PTS	+/PTS1 (SKL)	+/PTS1 (AKL)	nd	nd	nd
ECH	+/PTS1 (AKL)	+/PTS1 (SKL)	+/no PTS	+/PTS1 (AKL)	+/PTS1 (AKL)	+/PTS1 (ARM)	nd	nd	nd
3HCDH	+/PTS1 (SRL)	+/PTS1 (SRL)	+/PTS1 (SKL)	+/PTS1 (SKL)	+/PTS1 (SRL)	+/no PTSb	nd	nd	nd
ECI	+/PTS1 (PKL)	+/PTS1 (PKL)	+/PTS1 (SKM)	+/PTS1 (SRF)	+/PTS1 (PKL)	+/PTS1 (SNL)	nd	nd	nd
DECR	+/PTS1 (SRL)	+/PTS1 (SRL)	+/no PTSb	+/PTS1 (SSL)	+/PTS1 (SRL)	+/PTS1 (AKL)	nd	nd	nd
3KCT	+/PTS2 (RLTTLSGQF)	+/PTS2 (RLTTLSGQF)	+/PTS2? (RLVLGVVAL)	+/PTS2? (RLQAVTRQV)	+/PTS2 (RLTTLSGQF)	+/no PTSb	nd	nd	nd
CAT	+/PTS1 (AKM)	+/PTS1 (AKM)	+/no PTSb	+/PTS1 (ARM)	+/PTS1 (AKM)	+/PTS1 (SKM)	nd	nd	+/no PTS

Note.—Enzyme abbreviations: FACS: fatty acyl-CoA synthetase; ACOX: acyl-CoA oxidase; ECH: enoyl-CoA hydratase; 3HCDH: (3R)-hydroxyacyl-CoA dehydrogenase; ECI: enoyl-CoA isomerase; DECR: 2, 4-dienoyl-CoA reductase; 3KCT: 3-ketoacyl-CoA thiolase; CAT: catalase. See supplementary table S2, Supplementary Material online, for protein identification numbers. Organism name (and strain) abbreviations: Tg: Toxoplasma gondii ME49; Nc: Neospora caninum Liverpool; Et: Eimeria tenella Houghton; Cc: Cyclospora cayetanensis CHN_HEN01; Hh: Hammondia hammondi H.H.34; Sn: Sarcocystis neurona SO SN1; Pf: Plasmodium falciparum 3D7; Cp: Cryptosporidium parvum Iowa II; Gn: Gregarina niphandrodes. Other abbreviations: +: Ortholog present; nd: not detected; PTS: peroxisomal targeting signal type 1 or 2; SP: signal peptide; no PTS: no PTS predicted.

^aBest hit (blastP).

^bProtein sequence probably incomplete. PTS prediction not possible.