Figure 1.

DtxR TFs are required for normal growth under iron starvation and replete conditions. (A) Growth potential (area under the log-transformed growth curve, AUC) measurement of DtxR mutant strains compared to the isogenic parent under iron replete (blue bars) compared to iron starvation (orange bars) conditions. Each bar represents the data from the average of three independent biological replicates, each with three technical replicates. Error bars represent standard error of the mean (SEM). Stars represent P-value < 0.05 based on t-tests comparing the growth of the parent strain versus each mutant under the same iron condition (P-values listed in Supplementary Table S3). (B) Viable count survival assay. Each drop represents a 10-fold dilution, starting with undiluted on the left. The assay was performed in triplicate for each strain, with a representative plate shown in the figure, and the other two biological replicates shown in Supplementary Figure S1.